The Basic Principles Of high performance liquid chromatography
The Basic Principles Of high performance liquid chromatography
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Separation: The mobile section interacts With all the stationary section while in the column and also the analytes from the sample. This conversation influences how rapidly Each individual analyte travels throughout the column, leading to their separation.
The column dimension is the same. The column is filled with silica particles which are modified to make them non-polar. This is carried out by attaching lengthy hydrocarbon chains (eight–eighteen C atoms) to its area.
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). Since the tubing and fittings that have the cellular stage have stress limits, a higher back again stress requires a lower move charge and an extended Investigation time. Monolithic columns, by which the stable aid is an individual, porous rod, provide column efficiencies such as a packed capillary column whilst enabling for speedier circulation premiums. A monolithic column—which generally is comparable in dimensions to a conventional packed column, Despite the fact that smaller, capillary columns also are offered—is prepared by forming the mono- lithic rod inside a mold and covering it with PTFE tubing or possibly a polymer resin.
The selection with website the column form will depend on the physicochemical Attributes in the analytes currently being separated.
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규제 약물(마약, 합성 마약, 대마, 각성제, 향정신성 의약품, 아편양제제 등), 반도핑 관련(금지 물질, 금지 약물, 스테로이드 등), 약물 대사물
高速液体クロマトグラフィーにおいては各物質は比較的鋭いピークとして検出され、分離（他の物質のピークと明確に分けられる）および検出（鋭いピークにより高い感度が得られる）の能力が従来の液体クロマトグラフィーより良くなる。
The info acquisition system data and processes the indicators from the detector, allowing for with the development of chromatograms plus the quantification of compounds.
원하는 분석 결과를 얻기 위해서는 컬럼도 충분히 고려하고 선택하는 것이 좋습니다.
- 분석물의 분리여부는 고정상(컬럼)과 이동상의 조합에 의해 결정합니다.(실제 시료 측정에서는 시료 중에 분석물 이외의 오염물질에 존재하는 경우가 많아 분석자는 그 시료의 측정에 최적인 분석 조건의 검토가 필요합니다.
Degassing is attained in a number of approaches, but the most common are using a vacuum pump or sparging using how HPLC works an inert gasoline, for example He, which has a reduced solubility during the mobile stage. Particulate products, which can clog the HPLC tubing or column, are taken off by filtering the solvents.
The Examination is difficult by the advanced matrix of serum samples. A solid-stage extraction followed by an HPLC Investigation employing a fluorescence detector offers the required selectivity and detection limitations.
The separation of the individual parts during the mixture normally takes put inside the stationary phase from the column. In place of the glass column, it is ready in chrome steel.